ANTIBIOFILM ACTIVITY AND COMPOSITION OF THE PETROLEUM ETHER SOLUBLE FRACTION OF PSIDIUM GUAJAVA LINN.
Medicinal plants are considered as safe, significant and effective antimicrobial agents worldwide. The objective of present study is to determine antibiofilm potential and chemical composition of petroleum ether soluble fraction (PG-PES) of leaves extract of Psidium guajava Linn. (Guava; family Myrtaceae). Psidium guajava is a well known plant of tropical and subtropical region which is commonly used in various folkloric remedies. To the best of our knowledge, the scientific information on antibiofilm activity of non-polar fraction of this plant along with its chemical composition is first time reported. The antibiofilm activity was tested against seven human clinical pathogens with the comparison of standard drug ciprofloxacin. The strong antibiofilm effect was observed against Escherichia coli (84.5%), Staphylococcus aureus (84.0%), Pseudomonas aeruginosa (82.0%), Klebsiella pneumoniae (74.8%), Serratia marcescens (68.8%), while it showed moderate result against Streptococcus pyogenes (36.0%) and little effect against Staphylococcus epidermidis (7.9%).The chemical composition of this fraction identified the presence of seventeen compounds by GC/GC-MS analysis. The main constituents were D-friedoolean-14-en-3-one (13.75%), tert-butyl 1-[4-(2,6-ditert-butyl-4-methoxyphenoxy)-3-nitro-4-oxobutyl]pyrrolidine-2-carboxylate(13.15%),globulol (12.23%), 4,4,8-trimethyltricyclo[188.8.131.52(1,5)]dodecane-2,9-diol (11.22%) and isoaromadendrene epoxide (7.79 %).These investigations endorsed the incidence of various bioactive phyto constituents and also explored the use of PG-PES as an efficient, natural anti-biofilm agent against tested bacteria.