MOLECULAR CHARACTERIZATION OF AMYGDALUSSPINOSISSIMA COLLECTED FROM THE JUNIPER ECOSYSTEM OF BALOCHISTAN

Authors

  • RASHID ABDULLAH Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering and Management Sciences (BUITEMS), Quetta Pakistan.
  • SHAHJAHAN SHABBIR AHMED RANA Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering and Management Sciences (BUITEMS), Quetta Pakistan.
  • IFTEKHAR AHMED BALOCH Department of Botany, University of Turbat, Pakistan
  • NAZEER AHMED Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering and Management Sciences (BUITEMS), Quetta Pakistan.
  • IMRAN ALI SANI Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering and Management Sciences (BUITEMS), Quetta Pakistan.
  • SAADULLAH KHAN Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Enginee1ring and Management Sciences (BUITEMS), Quetta Pakistan.
  • DAWOOD SHAHID Department of Biotechnology, Faculty of Life Sciences, Balochistan University of Information Technology Engineering and Management Sciences (BUITEMS), Quetta Pakistan.
  • SAADULLAH LEGHARI Department of Botany, University of Balochistan, Pakistan

Abstract

Amygdalus spinosissima (Bunge) French. belongs to the family Rosaceae and is considered among the medicinal plants found in the juniper ecosystem of Balochistan. The technique of DNA barcoding was implied to identify several species, as it offers a reliable tool for molecular characterization. In this study two barcoding markers rbcL and matK were evaluated for the identification of A. spinosissima. The Determination of base substitutions, assessment of identified substitutions, and construction of phylogenetic trees using the Neighbor-Joining (NJ) method under the K2P distance model were carried out. Our study revealed 3 unique substitutions in rbcL samples and a single unique base substitution in matK samples. Based on phylogenetic analysis and sequence alignment we reportrbcL to be a more significant barcode maker as compared to matK for the identification of A. spinosissima.

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Published

2020-12-24