MOLECULAR IDENTIFICATION OF PARASITIC MISTLETOE (ARCEUTHOBIUM OXYCEDRI) OF JUNIPER ECOSYSTEM FROM DISTRICT ZIARAT, BALOCHISTAN, PAKISTAN

Authors

  • DAWOOD SHAHID Department of Biotechnology (FLS&I), Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Balochistan, Pakistan
  • NAZEER AHMED Department of Biotechnology (FLS&I), Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Balochistan, Pakistan
  • SHAHJAHAN SHABBIR AHMED Department of Biotechnology (FLS&I), Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Balochistan, Pakistan
  • IMRAN ALI SANI Department of Biotechnology (FLS&I), Balochistan University of Information Technology, Engineering and Management Sciences (BUITEMS), Quetta, Balochistan, Pakistan
  • MUHAMMAD FAHEEM SIDDIQUI Department of Botany, University of Karachi, Karachi 75270, Pakistan
  • ASIF ALI Department of Botany, University of Karachi, Karachi 75270, Pakistan
  • SHAZIA IRFAN Sardar Bahadur Khan Women University (SBK), Quetta, Balochistan, Pakistan
  • SAADULLAH LEGHARI Department of Botany, University of Balochistan, Quetta, Pakistan
  • SAADULLAH LEGHARI Department of Botany, University of Balochistan, Quetta, Pakistan
  • Asif Ur Rehman Mango Research Institute, Multan, Pakistan

Abstract

DNA barcoding is very effective tool in discriminating plants. Parasitic plants are responsible for destruction of plant locality. Arceuthobium oxycedri is a major cause of decline of Juniper forest Ziarat. There was no record of molecular data regarding this species in NCBI and GENBANK. Three markers i.e. RbcL, ITS-2 and Mat-k are used globally to obtain molecular data. CTAB method is used to extract DNA of eight A.oxycedri leaves from different areas of Sasnamanna, Ziarat. RbcL PCR amplification is 100% while Mat-k and ITS-2 were not amplified. RbcL discriminated Arceuthobium oxycedri at genus level as there is no data available in GENBANK and BOLD. 11 SNPs and 3 gaps found when compare A.oxycedri with Arceuthobium azoricum. In this research we have found that RbcL is much better primer than Mat-k and ITS-2 for identification of Arceuthobium oxycedri.

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Published

2021-06-25