ANTIOXIDANT PROPERTIES AND PHYTOCHEMICAL PRINCIPLES OF SOME MEDICINAL PLANTS: ADENIUM OBESUM (FORSSK.) ROEM. & SCHULT., IXORA COCCINEA LINN. AND AEGLE MARMELOS LINN.

Authors

  • AMNA KHATOON Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan
  • MUHAMMAD ALI VERSIANI Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan
  • SHUMAILA SHAMSHAD Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan
  • SAMINA ABDUL SATTAR Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan
  • HUMA KHAN Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Abstract

The methanolic extracts, fractions and sub-fractions of Adenium obesum (Forssk.) Roem. & Schult., Ixora coccinea Linn. and Aegle marmelos Linn. were screened for their free radical scavenging ability by using ascorbic acid and butylated hydroxytoulene (BHT) as standard antioxidants and evaluated through DPPH free radical scavenging assay. I. coccinea exhibited strongest inhibition, followed by A. obseum, and A. marmelos. The methanolic extracts of I. coccinea leaves proved to be the most potent antioxidant among these extracts with IC50 0.00315 ± 0.0001 mg/mL. Upon the purification of the active fractions twenty-six (1-26) pure chemical constituents were isolated and examined for their antioxidant inhibition ability, out of which 5-O-caffeoyl quinic acid (19) isolated from the active fraction of I. coccinea flower found to be potent antioxidant with IC50 value 0.0467 ± 0.0018 mg/mL as compared to standard ascorbic acid (0.0491 ± 0.0009) and BHT (0.0407 ± 0.003).

Author Biographies

AMNA KHATOON, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Department of Chemistry

MUHAMMAD ALI VERSIANI, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Department of Chemistry

SHUMAILA SHAMSHAD, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Department of Chemistry

SAMINA ABDUL SATTAR, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Department of Chemistry

HUMA KHAN, Federal Urdu University of Arts, Sciences and Technology, Gulshan-e- Iqbal, Science Campus, Karachi-75300, Pakistan

Department of Chemistry

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Published

2018-06-03