OPTIMAL CULTURAL CONDITIONS FOR INDUSTRIAL ENZYME PRODUCTION BY USING SHAKEN FLASK TECHNIQUE OF SUBMERGED FERMENTATION

Abstract

In this work optimization parameters in submerged fermentation were studied for the production of Invertase from Aspergillus flavus (Link 1809; IBGE 07) using agricultural based by-products (sunflower waste, cotton stalk, rice husk, date syrup and molasses) as sources of carbon. Effects of incubation time period (24-240 h), various cultural media (CM1, CM2, CM3, CM4 and CM5) sources of nitrogen (Corn steep, Casein, Potassium Nitrate Albumin Ammonium Sulphate Urea and Yeast Extract), pH (4.0-9.0), temperatures (30-70° C), inoculum size (4x106 -8x106 conidia) and agitation rate (50-300 rev/min) were also investigated for maximum invertase production. Optimal conditions for the production of invertase (7.41 U/mL) by Aspergillus flavus were observed when the strain was grown on culture medium CM1 containing yeast extract as a source of nitrogen, molasses as a source of carbon after 48 h of incubation at initial pH 6.5, temperature 40° C, inoculum size of 6x106 conidia in 50 mL of culture medium and agitation rate of 200 rev/min.