ANTIBACTERIAL ACTIVITY OF MARINE SPONGE COLLECTED FROM SUNHARI BEACH

Abstract

The antibacterial activity against eleven gram positive and seventeen gram negative bacteria were explored in this study. All the bacterial isolates were checked and identified on the basis of conventional methods for purity and maintained for further work. Antibacterial activity of crude extract of sponge against the test organisms were determined by using agar-well method. Autoclaved Muller Hinton broth was used to keep the bacterial culture in log phase for 2 hrs with constant agitation and subsequently wells were dug onto Muller Hinton Agar. All plates were incubated and after incubation diameter of zone of inhibition was measured.