A REVIEW ON TWO PHASE BATCH EXTRACTION OF ALKALINE PROTEASES

Abstract

Alkaline proteases are hydrolases that converts proteins into peptides performing better under low pH conditions. Numerous applications of alkaline proteases make them an important enzyme for industries. During conventional method of alkaline protease production, separate units are used for each phase of enzyme recovery, which is costly and time consuming. Extractive fermentation using aqueous two-phase system can reduce the time and cost for the production of alkaline proteases. Aqueous two-phase systems of interest in this review comprise polyethylene glycol (PEG) and potassium phosphate. Phase diagrams, factors and parameters affect the efficiency of two phase batch extraction of alkaline process.