ANTIMICROBIAL RESISTANCE PATTERN AGAINST POULTRY SALMONELLOSIS

Abstract

Salmonella enteritidis, which is mostly to blame for the bulk of Salmonella food-borne enteritis in people worldwide and may cause enteric infection in a range of host species, is the usual cause of poultry salmonellosis. This food-borne illness is a major problem in both industrialized and developing nations. The transmission of disease is facilitated by the presence of numerous animal reservoirs and the extensive commercial distribution of both livestock and food products. In terms of its effects on the socio-economic system, this disease is among the biggest public health issues. There was, however, little information available about their incidence, description, and antibiotic susceptibility in Pakistan. To lessen the public risk associated with consumption of contaminated items, S. enteritidis was identified in the current study using Whole cell protein profiling and plasmid encoded antibiotic resistance. Six antibiotics were used to evaluate ten isolates for plasmid-encoded antibiotic resistance. All isolates tested totally resistant to ampicillin (100%) in an in vitro susceptibility test using a typical disc, and that 60% of the isolates were resistant to amoxicillin. Levofloxacin and Ciprofloxacin were found sensitive to S. enteritidis i.e. 90% and 80% respectively. The plasmid profiling of resistant isolates revealed the presence of a plasmid that appears to be a serotype-specific virulence plasmid. By using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), whole cell protein profiling was carried out, revealing tight relationships between S. entertidis isolates and the presence of the main bands 78.1 and 35 kDa in all strains.