GENETIC STUDY OF MYP 2, MYP 15, MYP 16 AND MYP 17 LOCI IN MYOPIA AFFECTED FAMILIES IN THE PUNJAB

Abstract

Myopia is a common cause of impaired vision and visual disability and highly prevalent eye disease in Pakistan. It is multi factorial disease and 19 loci identified up to date. Seven myopic families were identified and selected for this study from different areas of Punjab province. Linkage analysis of these families was done by MYP2, MYP15, MYP16 and MYP17 loci (each consisting of a set of 3 microsatellite markers) of myopia that were selected from the panel of 19 loci. After DNA extraction and PCR amplification, linkage analysis was carried out by genotyping through PAGE and haplotypes were constructed for the families. This study leads us to find out the molecular basis of this disease for better understanding. Further study at broader scale with more number of families including more affected and normal individuals may identify any new locus involved.