IN VITRO CALLUS INDUCTION AND SHOOT FORMATION OF JUNIPERUS EXCELSA OF ZIARAT, BALOCHISTAN, PAKISTAN

Authors

  • SHAHBANO ALI KASHANI Institution of Biochemistry, University of Balochistan, Quetta, Pakistan
  • MUDASSIR ASRAR Institution of Biochemistry, University of Balochistan, Quetta, Pakistan
  • SAADULLAH KHAN LEGHARI Institution of Biochemistry, University of Balochistan, Quetta, Pakistan

Abstract

Effect of Activated Charcoal (AC), Yeast Extract (YE), L-glutamine (L-G), Polyethylene glycol (PEG), 6-Benzylaminopurine (BAP), 2,4-Dichlorphenoxyacetic acid (2,4-D), Indole-3-butyric acid (IBA) was checked to optimize protocol for in-vitro growth of Juniperus excelsa. The young plant shoots of J. excelsa were cultured in Woody Plant (WP) and Murashige and Skoogs (MS) media with five optimal concentration of different phyto-hormones (BAP, 2,4-D, IBA, AC, PEG 1000, YE and L-G). The results showed that BAP, 2,4-D, IBA and AC in both media showed statistically significant regeneration of root and shoot callus. However, PEG 1000 did not affect growth in both media and negatively affected at high concentration. YE and L-G enhanced growth of callus and shoot for 4 weeks in both media after that the callus and shoot formation stopped and no further growth was observed.

Author Biographies

SHAHBANO ALI KASHANI, Institution of Biochemistry, University of Balochistan, Quetta, Pakistan

Department of Botany, University of Balochistan, Quetta, Pakistan.

MUDASSIR ASRAR, Institution of Biochemistry, University of Balochistan, Quetta, Pakistan

Department of Botany, University of Balochistan, Quetta, Pakistan.

SAADULLAH KHAN LEGHARI, Institution of Biochemistry, University of Balochistan, Quetta, Pakistan

Department of Botany, University of Balochistan, Quetta, Pakistan.

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Published

2018-12-18

Issue

Vol. 8 No. 2 (2018): Volume 8 (2) December, 2018

Section

Articles

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