ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL OF LEAVES, BARK AND INFLORESCENCE OF IPOMOEA ERIOCARPA

Abstract

In present research antimicrobial and antioxidant potential of leaves, bark and inflorescence of Ipomoea eriocarpa R. Br. was assessed and maceration method was used for the formation of extracts, by using ethanol as solvent. Antimicrobial potential of I. eriocarpa was determined against different bacterial and fungal strains. The antimicrobial potential of I. eriocarpa ranges from 9.6 ± 1.2 mm to 6 ± 0.8 mm with maximum activity showed by ethanolic extract of bark against E. coli and minimum efficacy by ethanolic extract of inflorescence against B. subtilis as compared to the zone of inhibition of standard antibacterial discs Methicillin, i.e. 9.4 ± 1.22 and Amoxicillin, i.e.9.3 ± 1.30 mm. The A. oryzae strain showed more resistance towards the plant extracts in comparison to A. niger. The leaves of I. eriocarpa had exhibited activity against A. oryzae, i.e. 14.8 ± 0.3 mm and bark demonstrated activity against A. niger, i.e. 12.6 ± 1.2 mm. The inflorescence had showed maximum activity against A. oryzae, i.e.10.3±0.8 mm in comparison to antifungal disc Griseofulvin. Antioxidant potential of I. eriocarpa was evaluated by three methods, viz., Total phenolic content (TPC), Total flavonoids content (TFC) and DPPH (1, 1- Diphenyl-2-Picrylhydrazyl) radical scavenging activity. The TPC was evaluated and expressed as Quercetin ?g equiv. /mg of extract. The value of total flavonoid contents varied from 39.3 ± 0.85 to 1.14 ± 0.13µg/mg. The highest amount of flavonoids was found in the ethanolic extracts of leaves (39.3 ± 0.85 µg/mg). The value of % DPPH radical scavenging varied from 23.3 ± 0.03 to 76.5 ± 0.09% as compared to standard BHT, i.e. 87.7 ± 0.12%. These results revealed reasonable antimicrobial and antioxidant activity, thus supporting its medicinal worth.