MOLECULAR IDENTIFICATION OF PARASITIC MISTLETOE (ARCEUTHOBIUM OXYCEDRI) OF JUNIPER ECOSYSTEM FROM DISTRICT ZIARAT, BALOCHISTAN, PAKISTAN
DNA barcoding is very effective tool in discriminating plants. Parasitic plants are responsible for destruction of plant locality. Arceuthobium oxycedri is a major cause of decline of Juniper forest Ziarat. There was no record of molecular data regarding this species in NCBI and GENBANK. Three markers i.e. RbcL, ITS-2 and Mat-k are used globally to obtain molecular data. CTAB method is used to extract DNA of eight A.oxycedri leaves from different areas of Sasnamanna, Ziarat. RbcL PCR amplification is 100% while Mat-k and ITS-2 were not amplified. RbcL discriminated Arceuthobium oxycedri at genus level as there is no data available in GENBANK and BOLD. 11 SNPs and 3 gaps found when compare A.oxycedri with Arceuthobium azoricum. In this research we have found that RbcL is much better primer than Mat-k and ITS-2 for identification of Arceuthobium oxycedri.