EFFECT OF CHROMIUM AND ARSENIC ON GROWTH AND MORPHOLOGY OF MICE FETAL CALVARIAL CELLS (MFCC)

Abstract

Heavy metals are naturally present in environment but due to anthropogenic activities they are becoming pollutant. Naturally chromium is present in three oxidation states and its excessive amount is toxic and carcinogenic which lead to different cancers and other abnormalities. Drinking water contaminated with arsenic is big health problem all over the world and especially to developing countries. Mice calvarial cells were isolated from 18 day old fetus. Cell line was established from calvarial cells. Effect of chromium and arsenic was tested by applying different concentration of chromium and arsenic to cells. There is change in morphology of cells at higher chromium concentration, cells become round in shape and lysis of cells was also observed. Arsenic also proved to be very toxic for calvarial cells and there was complete change in morphology of cells at higher arsenic concentration. The effect of chromium and arsenic were more drastic with longer exposure time. In conclusion we can say that both chromium and arsenic are very toxic for mouse fetal calvarial cells.